Database Searching in Mass Spectrometry Based Proteomics

Title:Database Searching in Mass Spectrometry Based Proteomics

Volume: 7 Issue: 2

Author(s): Attila Kertesz-Farkas, Beata Reiz, Michael P. Myers and Sandor Pongor

Affiliation:

Keywords: Database search, Mass spectrometry, Protein identification, Proteomics, mass spectrum, Liquid chromatography, pattern recognition, genome annotation, 3D structure analysis), fragment

Abstract: Bottom-up proteomics (mass spectrometry analysis of peptides obtained by proteolysis and separated by liquid chromatography, (LC-MS/MS)) is one of the most frequently used techniques for identifying and characterizing proteins in biological samples. A key element of the analysis is database searching when the mass spectra of the peptides are compared with a database of theoretically computed (or experimental) peptide spectra. Here we discuss the main computational approaches to spectrum database searching and the statistical analysis of the results.

Cite this article as:

Kertesz-Farkas Attila, Reiz Beata, P. Myers Michael and Pongor Sandor, Database Searching in Mass Spectrometry Based Proteomics, Current Bioinformatics 2012; 7 (2) . https://dx.doi.org/10.2174/157489312800604354