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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

D-Serine-Dehydratase from Saccaromyces cerevisiae: A Pyridoxal 5’- phosphate-Dependent Enzyme for Advanced Biotech Applications

Author(s): Maria Staiano, Maria Strianese, Antonio Varriale, Stefano Di Giovanni, Donatella Scotto di Mase, Valentina Dell’Angelo, Giuseppe Ruggiero, Tullio Labella, Claudio Pellecchia and Sabato D’Auria

Volume 19, Issue 6, 2012

Page: [592 - 595] Pages: 4

DOI: 10.2174/092986612800494002

Price: $65

Abstract

The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-serine is presented. Our results show that this enzyme could be successfully used as a biological probe for detection of D-serine via fluorescence spectroscopy.

Keywords: Fluorescence, biosensors, D-Serine dehydratase, absorption, pyridoxal 5'-phosphate, fluorescence spectroscopy, NMDA (N-methyl-D-aspartate), D-serine, neuro-diseases, enzymatic assays


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