Proteomic Approaches to Understand Trichoderma Biocontrol Mechanisms and Plant Interactions

Rosa      Hermosa; Sheridan      L. Woo; Matteo      Lorito; Enrique      Monte

doi:10.2174/157016410793611783

Abstract

Trichoderma is an ascomycete fungal genus including species with a significant impact on agriculture and industrial processes. The first application of the proteome technology was carried out to extract and separate cell wall associated proteins of T. reesei. Following this work several studies performed on the proteome of different strains of T. atroviride, T. asperellum or T. harzianum interacting with pathogens and plants have provided many novel data that improve our understanding of the agricultural and biotechnological value of these fungi.

Keywords: Two-dimensional polyacrylamide gel electrophoresis, 2-D SDS-PAGE, mass spectrometry, MALDI-TOF-MS, liquid chromatography, LC-MS-MS, Trichoderma, Aspergillus, Botrytis, Neurospora, Penicillium, Phanerochaete, Pleurotus, Sclerotinia, Terebralia, Rhizoctonia solani, heat-shock proteins, Magnaporthe grisea, phosphoesterase, phosphocholine cytidyltransferase, glycosil hydrolase, endopolygalacturonase, Aspergillus niger, chitinase, xylanase, 1,3-glucanase, 1,6 glucanase, mannanase, glycoside hydrolases, endochitinase, Macrophomina phaseolina, MALDI-TOF/TOF-MS, glutathione peroxidase-like protein, 1,4-benzoquinone reductase, HEX1, cyclophilin A