Abstract
Zymography, the detection of enzymatic activity on gel electrophoresis, has been a technique described in the literature for at least in the past 50 years. Although a diverse amount of enzymes, especially proteases, have been detected, advances and improvements have been slower in comparison with other molecular biology, biotechnology and chromatography techniques. Most of the reviews and patents published focus on the technique as an element for enzymatic testing, but detailed analytical studies are scarce. Patents referring to zymography per se are few and the technique itself is hardly an important issue in titles or keywords in many scientific publications. This review covers a small condensation of the works published so far dealing with the identification of proteolytic enzymes in electrophoretic gel supports and its variations like 2-D zymography, real-time zymography, and in-situ zymography. Moreover, a scope will be given to visualize the new tendencies of this method, regarding substrates used and activity visualization. What to expect from zymography in the near future is also approached.
Keywords: Electrophoresis, enzymes, in-situ-zymography, matrix metallo-proteases, multiple-layer-zymography, proteases, two-dimensional zymography
Recent Patents on Biotechnology
Title: Protease Analysis by Zymography: A Review on Techniques and Patents
Volume: 3 Issue: 3
Author(s): Jeff Wilkesman and Liliana Kurz
Affiliation:
Keywords: Electrophoresis, enzymes, in-situ-zymography, matrix metallo-proteases, multiple-layer-zymography, proteases, two-dimensional zymography
Abstract: Zymography, the detection of enzymatic activity on gel electrophoresis, has been a technique described in the literature for at least in the past 50 years. Although a diverse amount of enzymes, especially proteases, have been detected, advances and improvements have been slower in comparison with other molecular biology, biotechnology and chromatography techniques. Most of the reviews and patents published focus on the technique as an element for enzymatic testing, but detailed analytical studies are scarce. Patents referring to zymography per se are few and the technique itself is hardly an important issue in titles or keywords in many scientific publications. This review covers a small condensation of the works published so far dealing with the identification of proteolytic enzymes in electrophoretic gel supports and its variations like 2-D zymography, real-time zymography, and in-situ zymography. Moreover, a scope will be given to visualize the new tendencies of this method, regarding substrates used and activity visualization. What to expect from zymography in the near future is also approached.
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Cite this article as:
Wilkesman Jeff and Kurz Liliana, Protease Analysis by Zymography: A Review on Techniques and Patents, Recent Patents on Biotechnology 2009; 3 (3) . https://dx.doi.org/10.2174/187220809789389162
DOI https://dx.doi.org/10.2174/187220809789389162 |
Print ISSN 1872-2083 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-4012 |
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