Recent Advances in Controlled Immobilization of Proteins onto the Surface of the Solid Substrate and Its Possible Application to Proteomics

Title: Recent Advances in Controlled Immobilization of Proteins onto the Surface of the Solid Substrate and Its Possible Application to Proteomics

Volume: 5 Issue: 3

Author(s): Kazuhiro Nakanishi, Takaharu Sakiyama, Yoichi Kumada, Koreyoshi Imamura and Hiroyuki Imanaka

Affiliation:

Keywords: Controlled immobilization, site-directed immobilization, proteome analysis, affinity peptide, ELISA, proteinprotein interaction

Abstract: Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of researchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while retaining structure and native biological function. In this review, we focus on recent advances in immobilization methodology for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library systems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.

Cite this article as:

Nakanishi Kazuhiro, Sakiyama Takaharu, Kumada Yoichi, Imamura Koreyoshi and Imanaka Hiroyuki, Recent Advances in Controlled Immobilization of Proteins onto the Surface of the Solid Substrate and Its Possible Application to Proteomics, Current Proteomics 2008; 5 (3) . https://dx.doi.org/10.2174/157016408785909622