Abstract
Histological assessment of nuclear morphology is a standard procedure in clinical practice. The implications of altered nuclear structure accompanying neoplastic disease are still not fully understood. Nuclear matrix proteins were considered as structural units defining the shape of nuclei. Consequently, it was assumed that nuclei displaying characteristics of cancer cells would exhibit specific nuclear matrix proteins. The nuclear proteome was investigated by various approaches, including analyses of preparations of whole nuclei, the nuclear matrix and of subnuclear compartments. Proteome alterations related to cancer were mainly investigated by comparative two dimensional polyacrylamide gel analyses. This review provides an overview of results achieved by this and other proteome analysis approaches, highlighting some implications of technical and analytical details. The contribution and future potential of proteomics to the further understanding of the chromatin regulation and mechanisms affecting nuclei during cell transformation will be discussed with regard to important advances accomplished by current techniques.
Keywords: nuclear matrix protein, cancer, proteomics, 2-d page, mass spectrometry
Current Proteomics
Title: Characteristic Alterations of Nuclear Structure and Chromatin Organisation of Cancer Cells Addressed by Proteome Analysis**
Volume: 1 Issue: 2
Author(s): Christopher Gerner
Affiliation:
Keywords: nuclear matrix protein, cancer, proteomics, 2-d page, mass spectrometry
Abstract: Histological assessment of nuclear morphology is a standard procedure in clinical practice. The implications of altered nuclear structure accompanying neoplastic disease are still not fully understood. Nuclear matrix proteins were considered as structural units defining the shape of nuclei. Consequently, it was assumed that nuclei displaying characteristics of cancer cells would exhibit specific nuclear matrix proteins. The nuclear proteome was investigated by various approaches, including analyses of preparations of whole nuclei, the nuclear matrix and of subnuclear compartments. Proteome alterations related to cancer were mainly investigated by comparative two dimensional polyacrylamide gel analyses. This review provides an overview of results achieved by this and other proteome analysis approaches, highlighting some implications of technical and analytical details. The contribution and future potential of proteomics to the further understanding of the chromatin regulation and mechanisms affecting nuclei during cell transformation will be discussed with regard to important advances accomplished by current techniques.
Export Options
About this article
Cite this article as:
Gerner Christopher, Characteristic Alterations of Nuclear Structure and Chromatin Organisation of Cancer Cells Addressed by Proteome Analysis**, Current Proteomics 2004; 1 (2) . https://dx.doi.org/10.2174/1570164043379406
DOI https://dx.doi.org/10.2174/1570164043379406 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Metabolomics and the Diagnosis of Human Diseases -A Guide to the Markers and Pathophysiological Pathways Affected
Current Medicinal Chemistry Potential Role of <i>In Vitro-In Vivo</i> Correlations (IVIVC) for the Development of Plant-Derived Anticancer Drugs
Current Drug Targets Iron Chelators in Cancer Chemotherapy
Current Topics in Medicinal Chemistry Heme Oxygenase-1 in Tumor Biology and Therapy
Current Drug Targets Targeted Alpha Therapy with 227Th-trastuzumab of Intraperitoneal Ovarian Cancer in Nude Mice
Current Radiopharmaceuticals Recent Developments in Chimeric NSAIDs as Anticancer Agents: Teaching an Old Dog a New Trick
Mini-Reviews in Medicinal Chemistry Recent Progress in the Development of Natural ent-Kaurane Diterpenoids with Anti-tumor Activity
Mini-Reviews in Medicinal Chemistry Genomic Signatures for Individualized Treatment of Malignant Tumors
Current Drug Discovery Technologies The Synergistic Effects of DNA-Targeted Chemotherapeutics and Histone Deacetylase Inhibitors As Therapeutic Strategies for Cancer Treatment
Current Medicinal Chemistry Induction of Tumour Cell Senescence: A New Strategy in Anticancer Treatment
Medicinal Chemistry Reviews - Online (Discontinued) Radiotracers in Oncology
Current Radiopharmaceuticals Cell Hierarchy, Metabolic Flexibility and Systems Approaches to Cancer Treatment
Current Pharmaceutical Biotechnology Intracellular Delivery of Potential Therapeutic Genes: Prospects in Cancer Gene Therapy
Current Gene Therapy Modulation of k-Ras Signaling by Natural Products
Current Medicinal Chemistry Impact of Metformin on Male Reproduction
Current Pharmaceutical Design A Systematic Review of Genes Involved in the Inverse Resistance Relationship Between Cisplatin and Paclitaxel Chemotherapy: Role of BRCA1
Current Cancer Drug Targets Statins and Alkylphospholipids as New Anticancer Agents Targeting Lipid Metabolism
Anti-Cancer Agents in Medicinal Chemistry Bladder Cancer Stem Cells
Current Stem Cell Research & Therapy Distinct Epigenetic Reprogramming, Mitochondrial Patterns, Cellular Morphology, and Cytotoxicity after Bee Venom Treatment
Recent Patents on Anti-Cancer Drug Discovery Estrogen Catechols Detection as Biomarkers in Schistosomiasis Induced Cancer and Infertility
Letters in Drug Design & Discovery