Abstract
Histological assessment of nuclear morphology is a standard procedure in clinical practice. The implications of altered nuclear structure accompanying neoplastic disease are still not fully understood. Nuclear matrix proteins were considered as structural units defining the shape of nuclei. Consequently, it was assumed that nuclei displaying characteristics of cancer cells would exhibit specific nuclear matrix proteins. The nuclear proteome was investigated by various approaches, including analyses of preparations of whole nuclei, the nuclear matrix and of subnuclear compartments. Proteome alterations related to cancer were mainly investigated by comparative two dimensional polyacrylamide gel analyses. This review provides an overview of results achieved by this and other proteome analysis approaches, highlighting some implications of technical and analytical details. The contribution and future potential of proteomics to the further understanding of the chromatin regulation and mechanisms affecting nuclei during cell transformation will be discussed with regard to important advances accomplished by current techniques.
Keywords: nuclear matrix protein, cancer, proteomics, 2-d page, mass spectrometry
Current Proteomics
Title: Characteristic Alterations of Nuclear Structure and Chromatin Organisation of Cancer Cells Addressed by Proteome Analysis**
Volume: 1 Issue: 2
Author(s): Christopher Gerner
Affiliation:
Keywords: nuclear matrix protein, cancer, proteomics, 2-d page, mass spectrometry
Abstract: Histological assessment of nuclear morphology is a standard procedure in clinical practice. The implications of altered nuclear structure accompanying neoplastic disease are still not fully understood. Nuclear matrix proteins were considered as structural units defining the shape of nuclei. Consequently, it was assumed that nuclei displaying characteristics of cancer cells would exhibit specific nuclear matrix proteins. The nuclear proteome was investigated by various approaches, including analyses of preparations of whole nuclei, the nuclear matrix and of subnuclear compartments. Proteome alterations related to cancer were mainly investigated by comparative two dimensional polyacrylamide gel analyses. This review provides an overview of results achieved by this and other proteome analysis approaches, highlighting some implications of technical and analytical details. The contribution and future potential of proteomics to the further understanding of the chromatin regulation and mechanisms affecting nuclei during cell transformation will be discussed with regard to important advances accomplished by current techniques.
Export Options
About this article
Cite this article as:
Gerner Christopher, Characteristic Alterations of Nuclear Structure and Chromatin Organisation of Cancer Cells Addressed by Proteome Analysis**, Current Proteomics 2004; 1 (2) . https://dx.doi.org/10.2174/1570164043379406
DOI https://dx.doi.org/10.2174/1570164043379406 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
An Update on Peptide Drugs for Voltage-Gated Calcium Channels
Recent Patents on CNS Drug Discovery (Discontinued) Mouse Strains for Prostate Tumorigenesis Based on Genes Altered in Human Prostate Cancer
Current Drug Targets Potential Therapeutic Benefits of Dipyridamole in COVID-19 Patients
Current Pharmaceutical Design The Association of Chemotherapy and Radiotherapy: Biological Rationale
Current Drug Therapy PI3K/Akt Signalling Pathway Specific Inhibitors: A Novel Strategy to Sensitize Cancer Cells to Anti-Cancer Drugs
Current Pharmaceutical Design Recent Nanocarrier Approaches for Targeted Drug Delivery in Cancer Therapy
Current Molecular Pharmacology CETUXIMAB: From Bench to Bedside
Current Cancer Drug Targets Immune Checkpoint Regulators: A New Era Toward Promising Cancer Therapy
Current Cancer Drug Targets Application of 3D Biomimetic Models in Drug Delivery and Regenerative Medicine
Current Pharmaceutical Design Combined Cancer Therapy with Non-Conventional Drugs: All Roads Lead to AMPK
Mini-Reviews in Medicinal Chemistry Is the Epithelial-to-Mesenchymal Transition Clinically Relevant for the Cancer Patient?
Current Pharmaceutical Biotechnology Bioactivities of Iridoids
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry TRPV1 Activation is Not An All-Or-None Event: TRPV1 Partial Agonism/Antagonism and Its Regulatory Modulation
Current Topics in Medicinal Chemistry Small Animal Computed Tomography Imaging
Current Medical Imaging NF-κB, a Potential Therapeutic Target for the Treatment of Multiple Sclerosis
CNS & Neurological Disorders - Drug Targets An Overview of Phytotherapeutic Approaches for the Treatment of Benign Prostatic Hyperplasia
Mini-Reviews in Medicinal Chemistry Vitamin D Metabolites and/or Analogs: Which D for Which Patient?
Current Vascular Pharmacology A Combined Approach with Rituximab Plus Anti-TRAIL-R Agonistic Antibodies for the Treatment of Haematological Malignancies
Current Pharmaceutical Design Targeted Delivery of Bleomycin: A Comprehensive Anticancer Review
Current Cancer Drug Targets Gene Electrotransfer: A Mechanistic Perspective
Current Gene Therapy