The Utility of Stable Cell Lines to Assess Species Differences in PXR Transactivation

Title: The Utility of Stable Cell Lines to Assess Species Differences in PXR Transactivation

Volume: 1 Issue: 2

Author(s): Michael W. Sinz, Devin Pray and Judy Raucy

Affiliation:

Keywords: Rat PXR, stable cells, CYP3A induction, toxicity, high volume screening

Abstract: A stable cell line was developed to assess activation of rat pregnane X receptor (rPXR) by xenobiotics. Characterization revealed that the greatest response occurred with dexamethasone (6-10 fold increase) while rifampicin failed to transactivate rPXR. Rodent PXR transactivation assays can be used to understand species differences in enzyme induction.

Cite this article as:

Sinz W. Michael, Pray Devin and Raucy Judy, The Utility of Stable Cell Lines to Assess Species Differences in PXR Transactivation, Drug Metabolism Letters 2007; 1 (2) . https://dx.doi.org/10.2174/187231207780363561