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Current HIV Research

Editor-in-Chief

ISSN (Print): 1570-162X
ISSN (Online): 1873-4251

Identification of HLA-A11-Restricted HIV-1-Specific Cytotoxic Tlymphocyte Epitopes in China

Author(s): Shaoyang Wang, Yongtao Sun, Song Zhai, Yan Zhuang, Shuguang Zhao, Whenzhen Kang, Xinhong Li, Dedong Huang, Xu G. Yu, Bruce D. Walker and Marcus A. Altfeld

Volume 5, Issue 1, 2007

Page: [119 - 128] Pages: 10

DOI: 10.2174/157016207779316404

Price: $65

Abstract

To fully define HLA-A11- restricted HIV-1- specific cytotoxic T-lymphocyte epitopes in China, a method combining the enzyme-linked immunospot (ELISPOT) assay with intracellular gamma interferon staining (ICS) of peripheral blood mononuclear cells (PBMC) was used to map the optimal epitopes targeted by ELISPOT and then to define the HLA restriction of epitopes by ICS. A novel HLA-A11-restricted CTL epitope and five other published HLA-A11-restricted epitopes previously identified by reverse immunogenetics or other methods were defined. The approach of integrating ELISPOT with ICS is both convenient and useful for the characterization of CTL responses to HIV-1 infection; this method is practical for defining novel epitopes and facilitates in developing new strategies for future vaccine design in China and other Asian countries.

Keywords: HIV, cytotoxic T-lymphocyte, epitopes, enzyme-linked immunospot (ELISPOT) assay, intracellular gamma interferon staining (ICS)


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