Identification of Heterotrimeric G Protein α and β Subunits in Rice

Title: Identification of Heterotrimeric G Protein α and β Subunits in Rice

Volume: 19 Issue: 3

Author(s): Yuki Abe, Keiko Matsusita, Setsuko Komatsu and Yukimoto Iwasaki

Affiliation:

Keywords: Antibody, Gα subunit, Gβ subunit, heterotrimeric G protein, purification, recombinant protein, affinity chromatography, polyvinylidene difluoride, Arabidopsis, centrifugation

Abstract: Like those in mammals, heterotrimeric G protein complexes have been implicated in signal transduction pathways in plants; however, the subunits themselves have not been isolated. In this study, the rice heterotrimeric G protein subunits α (Gα) and β (Gβ) were purified by affinity chromatography using anti-Gα and -Gβ antibodies and SDS-PAGE. Six and seven peptides, respectively, were identified by mass spectrometry and identified as the translation products of the Gα gene RGA1 and Gβ gene RGB1. During purification, the subunits dissociated easily from the G protein complex.

Cite this article as:

Abe Yuki, Matsusita Keiko, Komatsu Setsuko and Iwasaki Yukimoto, Identification of Heterotrimeric G Protein α and β Subunits in Rice, Protein & Peptide Letters 2012; 19 (3) . https://dx.doi.org/10.2174/092986612799363190

DOI https://dx.doi.org/10.2174/092986612799363190	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305