Abstract
A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and - dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.
Keywords: High-throughput screening, human umbilical cord blood mononuclear cells, natural products, flow cytometry, immuno-regulatory activities, γ-dodecalactone
Combinatorial Chemistry & High Throughput Screening
Title: A Screening Platform for Compounds with Potential Immuno-Regulatory Activities Using Human Cord Blood Mononuclear Cells
Volume: 9 Issue: 10
Author(s): Chia-Jung Chen, Chia Che Tsai, Jung-Feng Hsieh, Chichen Michael Chien, Tzu-Hua Wu and Shui-Tein Chen
Affiliation:
Keywords: High-throughput screening, human umbilical cord blood mononuclear cells, natural products, flow cytometry, immuno-regulatory activities, γ-dodecalactone
Abstract: A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and - dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.
Export Options
About this article
Cite this article as:
Chen Chia-Jung, Che Tsai Chia, Hsieh Jung-Feng, Michael Chien Chichen, Wu Tzu-Hua and Chen Shui-Tein, A Screening Platform for Compounds with Potential Immuno-Regulatory Activities Using Human Cord Blood Mononuclear Cells, Combinatorial Chemistry & High Throughput Screening 2006; 9 (10) . https://dx.doi.org/10.2174/138620706779026033
DOI https://dx.doi.org/10.2174/138620706779026033 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Colon Cancer and Specific Ways to Deliver Drugs to the Large Intestine
Anti-Cancer Agents in Medicinal Chemistry Voltage-Gated Sodium Channels as Therapeutic Targets for Treatment of Painful Diabetic Neuropathy
Mini-Reviews in Medicinal Chemistry Lifelong and Prenatal Effects of Phytoestrogens
Current Bioactive Compounds Type 3 Diabetes Mellitus: A Novel Implication of Alzheimers Disease
Current Topics in Medicinal Chemistry Phytoconstituents of <i>Lantana camara</i> L.: Rekindling Hope in the Cancer Treatment
Current Bioactive Compounds Pathophysiology of Preeclampsia and Possible Role of Zinc in its Genesis
Current Women`s Health Reviews Intra-uterine Growth Retardation as a Risk Factor of Postnatal Metabolic Disorders
Current Pharmaceutical Biotechnology Post-translational and Post-transcriptional Modifications of Pregnane X Receptor (PXR) in Regulation of the Cytochrome P450 Superfamily
Current Drug Metabolism Oocyte Cryopreservation for Elective Preservation of Reproductive Potential
Current Women`s Health Reviews Human Microdialysis
Current Pharmaceutical Biotechnology Ion Transporters in Brain Tumors
Current Medicinal Chemistry The ABCA2 Transporter: Intracellular Roles in Trafficking and Metabolism of LDL-Derived Cholesterol and Sterol-Related Compounds
Current Drug Metabolism Post-Translational Modifications of Natural Antimicrobial Peptides and Strategies for Peptide Engineering
Current Biotechnology Modulation of Neutrophil Function by Hormones
Current Immunology Reviews (Discontinued) LNA Antisense: A Review
Current Physical Chemistry Uncommon Becoming Common: Case Series of Primary Fallopian Tube Cancers
Current Women`s Health Reviews DNA Polymerases and Oxidative Damage: Friends or Foes?
Current Molecular Pharmacology Adjuvant Hormonal Therapy in Women with Early-stage Breast Cancer
Medicinal Chemistry Marine Derived Anticancer Drugs Targeting Microtubule
Recent Patents on Anti-Cancer Drug Discovery A Possible Placental Factor for Preeclampsia: sFlt-1
Current Medicinal Chemistry