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Current Protein & Peptide Science

Editor-in-Chief

ISSN (Print): 1389-2037
ISSN (Online): 1875-5550

Mass Spectrometry Characterization of Plant Phosphoproteins

Author(s): Katerina Novakova, Ondrej Sedo and Zbynek Zdrahal

Volume 12, Issue 2, 2011

Page: [112 - 125] Pages: 14

DOI: 10.2174/138920311795684913

Price: $65

Abstract

Protein phosphorylation constitutes a major type of post-translational modifications mobilizing a high number of genes. It is involved in many crucial cell processes and largely participates in the features of the proteome. For several biological and technical reasons, the characterization of protein phosphorylation requires a combination of distinct procedures. In this review, a special emphasis is given to analytical strategies connected with the determination of the presence of phosphorylation sites and their localization by mass spectrometry (MS). The feasibility of selected combinations of analytical approaches for diverse objectives of phosphoproteomic research is discussed.

Keywords: Plant phosphorylation, phosphoproteomics, mass spectrometry, sample preparation, post-translational modifications, monitored protein separation, kinase inhibitors, spectrometric analysis., Liquid chromatography, lyophilization, Gel Electrophoresis, Chemical Tagging, Phospho-specific visualization, immunopurification


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