Isolation and Detection of Proteins with Nano-Particles and Microchips for Analyzing Proteomes on a Large Scale Basis

Ki      Chan; Tzi      Bun Ng

doi:10.2174/092986611794653978

Abstract

The advent of sugar-immobilized gold nano-particles (SGNPs), lipid-based nanoparticles, nanochromatography and nano-electrophoresis has revolutionized the methodology for proteins purification and proteomic research. This review provides an overview on the effective method developed for fast purification of proteins from extracts using SGNPs. In addition, the current application of microfluidic systems for analytical purposes in biochemistry will also be explored that include the micro total analysis systems (μ-TAS) and lab-on-a-chip (LOC) analyses which are capable of isolation and detection of proteins at the nanogram level. Finally, we describe why the lipid-based nano-particles (LBNPs) can enable the analysis in microchip electroseparation and how anionic and cationic LBNPs can be used for proteins separation.

Keywords: Nanoparticles, micro-total-analysis systems, lab-on-a-chip, capillary electrochromatography, Microchips, Proteomes, sugar-immobilized gold nano-particles (SGNPs), nanochromatography, nano-electrophoresis, microfluidic systems, TAS, lab-on-a-chip (LOC), lipid-based nano-particles (LBNPs), Chromatography, CE equipmentNanoparticles, micro-total-analysis systems, lab-on-a-chip, capillary electrochromatography, Microchips, Proteomes, sugar-immobilized gold nano-particles (SGNPs), nanochromatography, nano-electrophoresis, microfluidic systems, TAS, lab-on-a-chip (LOC), lipid-based nano-particles (LBNPs), Chromatography, CE equipment