Abstract
Temperature dependence of the α-helix conformation of bee venom melittin in methanol-water mixed solvents has been examined by NMR, in order to elucidate conformation stability and a phase diagram. At high methanol concentration of 100 – ca. 80 wt.%, melittin forms a full α-helix conformation in the temperature range from 25 °C to 60 °C. At intermediate methanol concentration of ca. 80 – ca. 25 wt.%, it undergoes a thermal transformation from a full α-helix to a partial α-helix. In solutions of low methanol concentrations of ca. 25 – 0 wt.%, partial α-helix monomers and their selfaggregated conformers coexist at low temperatures, and the relative number of the monomers increases with increase in temperature. The monomers turn to a random coil state at high temperatures only below ca. 10 wt. % methanol concentrations. The thermal transitions are discussed from the viewpoint of stability of intra-molecular hydrogen bonds and intermolecular hydrophobic interactions.
Keywords: α-helix, hydrogen bond, hydrophobic interaction, melittin, methanol-water mixed solvent, NMR, phase diagramα-helix, hydrogen bond, hydrophobic interaction, melittin, methanol-water mixed solvent, NMR, phase diagram
Protein & Peptide Letters
Title: Helix Conformation of a Small Peptide Melittin in a Methanol-Water Mixed Solvent Studied by NMR
Volume: 18 Issue: 3
Author(s): Yoshinori Miura
Affiliation:
Keywords: α-helix, hydrogen bond, hydrophobic interaction, melittin, methanol-water mixed solvent, NMR, phase diagramα-helix, hydrogen bond, hydrophobic interaction, melittin, methanol-water mixed solvent, NMR, phase diagram
Abstract: Temperature dependence of the α-helix conformation of bee venom melittin in methanol-water mixed solvents has been examined by NMR, in order to elucidate conformation stability and a phase diagram. At high methanol concentration of 100 – ca. 80 wt.%, melittin forms a full α-helix conformation in the temperature range from 25 °C to 60 °C. At intermediate methanol concentration of ca. 80 – ca. 25 wt.%, it undergoes a thermal transformation from a full α-helix to a partial α-helix. In solutions of low methanol concentrations of ca. 25 – 0 wt.%, partial α-helix monomers and their selfaggregated conformers coexist at low temperatures, and the relative number of the monomers increases with increase in temperature. The monomers turn to a random coil state at high temperatures only below ca. 10 wt. % methanol concentrations. The thermal transitions are discussed from the viewpoint of stability of intra-molecular hydrogen bonds and intermolecular hydrophobic interactions.
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Cite this article as:
Miura Yoshinori, Helix Conformation of a Small Peptide Melittin in a Methanol-Water Mixed Solvent Studied by NMR, Protein & Peptide Letters 2011; 18 (3) . https://dx.doi.org/10.2174/092986611794578387
DOI https://dx.doi.org/10.2174/092986611794578387 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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