Characterization of Human Tyrosinase Ectodomain Expressed in Escherichia coli

Title: Characterization of Human Tyrosinase Ectodomain Expressed in Escherichia coli

Volume: 17 Issue: 8

Author(s): Ji-Na Kong, Hee-Jin Lee, Dong-Hyun Jo and Kwang-Hoon Kong

Affiliation:

Keywords: Biochemical properties, Ectodomain, Expression in Escherichia coli, Human tyrosinase

Abstract: The human tyrosinase ectodomain has been expressed in Escherichia coli as a soluble form and purified by immobilized metal affinity column chromatography. The ectodomain exhibited tyrosinase activities toward the hydroxylation and oxidation reactions. Biochemical properties of the ectodomain appeared to be distinct from those of the human tyrosinase, although common features were retained.

Cite this article as:

Kong Ji-Na, Lee Hee-Jin, Jo Dong-Hyun and Kong Kwang-Hoon, Characterization of Human Tyrosinase Ectodomain Expressed in Escherichia coli, Protein & Peptide Letters 2010; 17 (8) . https://dx.doi.org/10.2174/092986610791498957

DOI https://dx.doi.org/10.2174/092986610791498957	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305