Structural Disorder in Proteins of the Rhabdoviridae Replication Complex

Title: Structural Disorder in Proteins of the Rhabdoviridae Replication Complex

Volume: 17 Issue: 8

Author(s): Cedric Leyrat, Francine C.A. Gerard, Euripedes de Almeida Ribeiro Jr., Ivan Ivanov, Rob W.H. Ruigrok and Marc Jamin

Affiliation:

Keywords: Rhabdoviridae, replication complex, phosphoprotein, nucleoprotein, intrinsically disordered regions

Abstract: Rhabdoviridae are single stranded negative sense RNA viruses. The viral RNA condensed by the nucleoprotein (N), the phosphoprotein (P) and the large subunit (L) of the RNA-dependent RNA polymerase are the viral components of the transcription/replication machineries. Both P and N contain intrinsically disordered regions (IDRs) that play different roles in the virus life cycle. Here, we describe the modular organization of P based on recent structural, biophysical and bioinformatics data. We show how flexible loops in N participate in the attachment of P to the N-RNA template by an induced- fit mechanism. Finally, we discuss the roles of IDRs in the mechanism of replication/transcription, and propose a new model for the interaction of the L subunit with its N-RNA template.

Cite this article as:

Leyrat Cedric, C.A. Gerard Francine, de Almeida Ribeiro Jr. Euripedes, Ivanov Ivan, W.H. Ruigrok Rob and Jamin Marc, Structural Disorder in Proteins of the Rhabdoviridae Replication Complex, Protein & Peptide Letters 2010; 17 (8) . https://dx.doi.org/10.2174/092986610791498939

DOI https://dx.doi.org/10.2174/092986610791498939	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305