Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

Title: Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

Volume: 17 Issue: 3

Author(s): Lara Padovan, Ludovica Segat, Alessandro Tossi, Tercilio Calsa Jr., Akio Kido Ederson, Lucas Brandao, Rafael L. Guimaraes, Valesca Pandolfi, Maria Clara Pestana-Calsa, Luis Carlos Belarmino, Ana Maria Benko-Iseppon and Sergio Crovella

Affiliation:

Keywords: Antimicrobial peptides, cowpea mosaic virus, evolution, plant defensin, protein function, Vigna unguiculata

Abstract: Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna unguiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.

Cite this article as:

Padovan Lara, Segat Ludovica, Tossi Alessandro, Calsa Jr. Tercilio, Ederson Kido Akio, Brandao Lucas, Guimaraes L. Rafael, Pandolfi Valesca, Pestana-Calsa Clara Maria, Belarmino Carlos Luis, Benko-Iseppon Maria Ana and Crovella Sergio, Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.), Protein & Peptide Letters 2010; 17 (3) . https://dx.doi.org/10.2174/092986610790780350

DOI https://dx.doi.org/10.2174/092986610790780350	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305