Annotation of Non-Synonymous Single Polymorphisms in Human Liver Proteome by Mass Spectrometry

Title: Annotation of Non-Synonymous Single Polymorphisms in Human Liver Proteome by Mass Spectrometry

Volume: 17 Issue: 3

Author(s): Ming Chen, Bing Yang, Wantao Ying, Fuchu He and Xiaohong Qian

Affiliation:

Keywords: Liquid chromatography-tandem mass spectrometry, liver proteins, nsSNP, peptide database, SIFT

Abstract: A novel strategy to annotate nsSNP-peptides in human liver proteome based on LC-ESI-MS/MS and peptide database search was proposed. Totally 115 nsSNP-peptides in human liver proteins were annotated using our method. Among them, 42 peptides were found to be amino acid mutation, 73 peptides were wild type, 5 peptides were interpreted with both mutation and wild type. The function of nsSNP-peptide was predicted using SIFT algorithm, and 2 nsSNPs were predicted to be damaged for protein function. The results here show that the strategy is very effective for annotation of nsSNP at peptide level.

Cite this article as:

Chen Ming, Yang Bing, Ying Wantao, He Fuchu and Qian Xiaohong, Annotation of Non-Synonymous Single Polymorphisms in Human Liver Proteome by Mass Spectrometry, Protein & Peptide Letters 2010; 17 (3) . https://dx.doi.org/10.2174/092986610790780242

DOI https://dx.doi.org/10.2174/092986610790780242	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305