Effect of Protein Concentration and pH on the Chitinase Activity of Tapes japonica Lysozyme

Title: Effect of Protein Concentration and pH on the Chitinase Activity of Tapes japonica Lysozyme

Volume: 17 Issue: 2

Author(s): Takashi Goto, Yoshito Abe, Taiji Imoto and Tadashi Ueda

Affiliation:

Keywords: Invertebrate-type lysozyme, chitinase activity, dimer formation

Abstract: Tapes japonica lysozyme (TJL), which belongs to the invertebrate-type lysozyme family, has a unique dimer formation. The residues, which include catalytic residues (glutamate 18 and aspartate 30), at the dimer interface form electrostatic interactions. Our previous study suggested that increasing the NaCl concentration switched TJL from a dimer to monomer structure, which increased TJL activity. Therefore, conversion from the dimeric to the monomeric structure is crucial for the TJL activity. In the present study, to further understand the effect of NaCl on TJL dimer formation, we examined the protein concentration and pH dependence of TJL activity in the presence or absence of 500 mM NaCl. TJL activity was suppressed at the high protein concentration. And the optimum pH of TJL activity was decreased in the absence of NaCl. These dependencies confirm the presence of electrostatic interactions between molecules of TJL in the dimeric form in an aqueous solution.

Cite this article as:

Goto Takashi, Abe Yoshito, Imoto Taiji and Ueda Tadashi, Effect of Protein Concentration and pH on the Chitinase Activity of Tapes japonica Lysozyme, Protein & Peptide Letters 2010; 17 (2) . https://dx.doi.org/10.2174/092986610790226094

DOI https://dx.doi.org/10.2174/092986610790226094	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305