Functional Cloning and Expression of a Novel Endo-α-1,5-L-Arabinanase from a Metagenomic Library

Title: Functional Cloning and Expression of a Novel Endo-α-1,5-L-Arabinanase from a Metagenomic Library

Volume: 16 Issue: 12

Author(s): Dominic W.S. Wong, Victor J. Chan and Amanda A. McCormack

Affiliation:

Keywords: Arabinanase, endo-arabinanase, pectinase, arabinan-degrading enzyme

Abstract: A novel endo-α-L-arabinanase gene (arn2) was isolated, and expressed in E. coli in active form. The recombinant enzyme (ARN2) had optimum activity at pH 6.0 and 45-50oC with stability between pH 5.0-8.0 and at temperatures up to 40oC. The recombinant ARN2 catalyzed internal cleavage of α-1,5 glycosidic bonds of CM-arabinan, debranched arabinan, linear arabinan, and sugar beet (native) arabinan at rates of decreasing order, and was inactive on wheat arabinoxylan and p-nitrophenyl- α-L-arabinofuranoside. Kinetic analysis showed that branching in the arabinan did not significantly affect the apparent Km values, and the difference in the reaction rates was likely due to the chemical step after substrate binding. The enzyme hydrolyzed arabino-oligosaccharides of DP 6 to smaller oligomers and mostly arabinotriose. Natural and modified arabinans were cleaved to oligomers of various chain lengths, which were progressively hydrolyzed to yield arabinotriose. The pattern of degradation revealed an endo-acting mechanism with arabinotriose as the end product.

Cite this article as:

Wong W.S. Dominic, Chan J. Victor and McCormack A. Amanda, Functional Cloning and Expression of a Novel Endo-α-1,5-L-Arabinanase from a Metagenomic Library, Protein & Peptide Letters 2009; 16 (12) . https://dx.doi.org/10.2174/092986609789839313