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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Insulin-Binding Canavalin Is Present in Canavalia ensiformis Seed Coat

Author(s): Elane da Silva Ribeiro, Adriana Ferreira Uchoa, Leonardo Gomes da Silva, Daniela Gois Beghini, Andre Teixeira da Silva Ferreira, Jonas Perales, Tania Jacinto, Katia Valevski Sales Fernandes, Jose Xavier-Filho and Antonia Elenir Amancio Oliveira

Volume 16, Issue 9, 2009

Page: [1106 - 1111] Pages: 6

DOI: 10.2174/092986609789055377

Price: $65

Abstract

An insulin-binding protein was isolated from Canavalia ensiformis seed coat, by using an insulin-Sepharose 4B affinity chromatography, and the protein was identified as canavalin (Canavalia 7S globulin) by mass spectrometry analysis. The major novelty of these data is the acidic nature of this globulin insulin-binding, in contrast to the basic Bg-like insulin- binding proteins so far reported in plants.

Keywords: 7S globulins, canavalin, vicilin, insulin-binding proteins, mass spectrometry


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