Abstract
An insulin-binding protein was isolated from Canavalia ensiformis seed coat, by using an insulin-Sepharose 4B affinity chromatography, and the protein was identified as canavalin (Canavalia 7S globulin) by mass spectrometry analysis. The major novelty of these data is the acidic nature of this globulin insulin-binding, in contrast to the basic Bg-like insulin- binding proteins so far reported in plants.
Keywords: 7S globulins, canavalin, vicilin, insulin-binding proteins, mass spectrometry
Protein & Peptide Letters
Title: Insulin-Binding Canavalin Is Present in Canavalia ensiformis Seed Coat
Volume: 16 Issue: 9
Author(s): Elane da Silva Ribeiro, Adriana Ferreira Uchoa, Leonardo Gomes da Silva, Daniela Gois Beghini, Andre Teixeira da Silva Ferreira, Jonas Perales, Tania Jacinto, Katia Valevski Sales Fernandes, Jose Xavier-Filho and Antonia Elenir Amancio Oliveira
Affiliation:
Keywords: 7S globulins, canavalin, vicilin, insulin-binding proteins, mass spectrometry
Abstract: An insulin-binding protein was isolated from Canavalia ensiformis seed coat, by using an insulin-Sepharose 4B affinity chromatography, and the protein was identified as canavalin (Canavalia 7S globulin) by mass spectrometry analysis. The major novelty of these data is the acidic nature of this globulin insulin-binding, in contrast to the basic Bg-like insulin- binding proteins so far reported in plants.
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Ribeiro da Silva Elane, Uchoa Ferreira Adriana, da Silva Gomes Leonardo, Beghini Gois Daniela, Ferreira Teixeira da Silva Andre, Perales Jonas, Jacinto Tania, Sales Fernandes Valevski Katia, Xavier-Filho Jose and Oliveira Elenir Amancio Antonia, Insulin-Binding Canavalin Is Present in Canavalia ensiformis Seed Coat, Protein & Peptide Letters 2009; 16 (9) . https://dx.doi.org/10.2174/092986609789055377
DOI https://dx.doi.org/10.2174/092986609789055377 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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