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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli

Author(s): Thomas Vargues, Gareth J. Morrison, Emily S. Seo, David J. Clarke, Helen L. Fielder, Julien Bennani, Uday Pathania, Fiona Kilanowski, Julia R. Dorin, John R. W. Govan, C. Logan Mackay, Dusan Uhrin and Dominic J. Campopiano

Volume 16, Issue 6, 2009

Page: [668 - 676] Pages: 9

DOI: 10.2174/092986609788490122

Price: $65

Abstract

Human β-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 β-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed H NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.

Keywords: HBD2, Defensin, Peptide, E. coli, Chemotaxis, Antimicrobial activity


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