Analysis of Pralidoxime in Serum, Brain and CSF of Rats

Title: Analysis of Pralidoxime in Serum, Brain and CSF of Rats

Volume: 5 Issue: 3

Author(s): Huba Kalasz, Eva Szoko, Tamas Tabi, Georg A. Petroianu, Dietrich E. Lorke, Abdulrab Omar, Salem Alafifi, Almerri Jasem and Kornelia Tekes

Affiliation:

Keywords: CZE, pralidoxime, serum, cerebrospinal fluid (CSF), brain

Abstract: After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 – 200 μg/mL, 0.3 – 7 μg/mL and 0.1 – 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoximetype antidotes clinically used in organophosphate poisoning.

Cite this article as:

Kalasz Huba, Szoko Eva, Tabi Tamas, Petroianu A. Georg, Lorke E. Dietrich, Omar Abdulrab, Alafifi Salem, Jasem Almerri and Tekes Kornelia, Analysis of Pralidoxime in Serum, Brain and CSF of Rats, Medicinal Chemistry 2009; 5 (3) . https://dx.doi.org/10.2174/157340609788185882