Facile Conversion of FP to TR-FRET Assays using Terbium Chelates:Nuclear Receptor Competitive Binding Assays as Examples

Title: Facile Conversion of FP to TR-FRET Assays using Terbium Chelates:Nuclear Receptor Competitive Binding Assays as Examples

Volume: 5 Issue: 6

Author(s): Kurt W. Vogel, Bryan D. Marks, Kevin R. Kupcho, Kevin L. Vedvik and Tina M. Hallis

Affiliation:

Keywords: Fluorescence polarization, Time-resolved fret, Terbium chelates, Nuclear receptors

Abstract: Time-resolved FRET (TR-FRET) assays offer advantages over fluorescence polarization (FP) assays including reduced optical interference from compound autofluorescence or precipitation, and a greater ability to resolve potencies of tight-binding ligands. Here, we demonstrate the facile conversion of FP assays to a TR-FRET format using nuclear receptor ligand binding assays as examples.

Cite this article as:

Vogel W. Kurt, Marks D. Bryan, Kupcho R. Kevin, Vedvik L. Kevin and Hallis M. Tina, Facile Conversion of FP to TR-FRET Assays using Terbium Chelates:Nuclear Receptor Competitive Binding Assays as Examples, Letters in Drug Design & Discovery 2008; 5 (6) . https://dx.doi.org/10.2174/157018008785777261