Abstract
The Antarctic eubacterium Pseudoalteromonas haloplanktis (Ph) produces a cold-active iron superoxide dismutase (SOD). PhSOD is a homodimeric enzyme, that displays a high catalytic activity even at low temperature. Using hanging-drop vapour-diffusion technique, PhSOD has been successfully crystallized in two different crystal forms. Both crystal forms are monoclinic with space group P21 and diffract to 2.1 Å resolution. Form I has unit-cell parameters a=45.49Å b=103.63Å c=50.37Å β=108.2° and contains a homodimer in the asymmetric unit. Form II has unit-cell parameters a=50.48Å b=103.78Å c=90.25Å β=103.8° and an asymmetric unit containing two PhSOD homodimers. Structure determination has been achieved using molecular replacement. The crystallographic study of this cold-adapted enzyme could contribute to the understanding of the molecular mechanisms of cold-adaptation and of the high catalytic efficiency at low temperature.
Keywords: Iron superoxide dismutase, Pseudoalteromonas haloplanktis, crystallization, cold adaptation, X-ray
Protein & Peptide Letters
Title: Crystallization and Preliminary X-Ray Diffraction Studies of a Psychrophilic Iron Superoxide Dismutase from Pseudoalteromonas haloplanktis
Volume: 15 Issue: 4
Author(s): Antonello Merlino, Irene Russo Krauss, Immacolata Castellano, Emmanuele De Vendittis, Alessandro Vergara and Filomena Sica
Affiliation:
Keywords: Iron superoxide dismutase, Pseudoalteromonas haloplanktis, crystallization, cold adaptation, X-ray
Abstract: The Antarctic eubacterium Pseudoalteromonas haloplanktis (Ph) produces a cold-active iron superoxide dismutase (SOD). PhSOD is a homodimeric enzyme, that displays a high catalytic activity even at low temperature. Using hanging-drop vapour-diffusion technique, PhSOD has been successfully crystallized in two different crystal forms. Both crystal forms are monoclinic with space group P21 and diffract to 2.1 Å resolution. Form I has unit-cell parameters a=45.49Å b=103.63Å c=50.37Å β=108.2° and contains a homodimer in the asymmetric unit. Form II has unit-cell parameters a=50.48Å b=103.78Å c=90.25Å β=103.8° and an asymmetric unit containing two PhSOD homodimers. Structure determination has been achieved using molecular replacement. The crystallographic study of this cold-adapted enzyme could contribute to the understanding of the molecular mechanisms of cold-adaptation and of the high catalytic efficiency at low temperature.
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Cite this article as:
Merlino Antonello, Krauss Russo Irene, Castellano Immacolata, Vendittis De Emmanuele, Vergara Alessandro and Sica Filomena, Crystallization and Preliminary X-Ray Diffraction Studies of a Psychrophilic Iron Superoxide Dismutase from Pseudoalteromonas haloplanktis, Protein & Peptide Letters 2008; 15 (4) . https://dx.doi.org/10.2174/092986608784246533
DOI https://dx.doi.org/10.2174/092986608784246533 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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