Abstract
A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.
Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast
Protein & Peptide Letters
Title: Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris
Volume: 15 Issue: 4
Author(s): C. P.S. Carvalho, C. S. Rocha, D. R. Nepomuceno, J. T.A. Oliveira and T. B. Grangeiro
Affiliation:
Keywords: Recombinant protein, carbohydrate-binding, phytohemagglutinin, yeast
Abstract: A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.
Export Options
About this article
Cite this article as:
Carvalho P.S. C., Rocha S. C., Nepomuceno R. D., Oliveira T.A. J. and Grangeiro B. T., Expression of a Canavalia brasiliensis Lectin (ConBr) Precursor in Pichia pastoris, Protein & Peptide Letters 2008; 15 (4) . https://dx.doi.org/10.2174/092986608784246579
DOI https://dx.doi.org/10.2174/092986608784246579 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Allergy and Inflammation: An Immunological and Therapeutic Approach
Recent Patents on Inflammation & Allergy Drug Discovery Phosphoinositide 3-Kinases and Leukocyte Migration
Current Immunology Reviews (Discontinued) Role of the Akt Pathway in Prostate Cancer
Current Cancer Drug Targets Recent Progress on Tumor Missile Therapy and Tumor Vascular Targeting Therapy as a New Approach
Current Vascular Pharmacology Tumor Vasculature Targeting Through NGR Peptide-Based Drug Delivery Systems
Current Pharmaceutical Biotechnology New Approaches in Glioblastoma Multiforme: The Potential Role of Immune- check Point Inhibitors
Current Cancer Drug Targets Immunomodulatory and Anti-Inflammatory Activities of Statins
Endocrine, Metabolic & Immune Disorders - Drug Targets The Rapidly Changing Composition of the Global Street Drug Supply and its Effects on High-risk Groups for COVID-19
Current Psychopharmacology The Multifunctional Anti-inflammatory Drugs Used in the Therapy of Alzheimer’s Disease
Current Medicinal Chemistry Anti-Inflammatory Properties of Acetylcholinesterase Inhibitors Administred in Alzheimers Disease
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Significance of P2X7 Receptor Variants to Human Health and Disease
Recent Patents on DNA & Gene Sequences Early Growth Response-1 (EGR-1) – A Key player in Myocardial Cell Injury
Cardiovascular & Hematological Agents in Medicinal Chemistry Intracellular ROS Generation Mediates Maleimide-induced Cytotoxicity in Leukemia Cells
Anti-Cancer Agents in Medicinal Chemistry Pseudomonas aeruginosa Invades Human Aortic Endothelial Cells and Induces Cell Damage in vitro
Cardiovascular & Hematological Disorders-Drug Targets Herpesvirus Saimiri-Based Gene Delivery Vectors
Current Gene Therapy Editorial [Hot Topic: Immunomodulators in Medicinal Chemistry and Drug Discovery (Guest Editor: Dr. Athanasia Mouzaki)]
Current Topics in Medicinal Chemistry The Stem Cell Factor Receptor/c-Kit as a Drug Target in Cancer
Current Cancer Drug Targets Novel Drugs for Neuroblastoma
Drug Design Reviews - Online (Discontinued) The Role of Angiotensin in Obesity and Metabolic Disease
Endocrine, Metabolic & Immune Disorders - Drug Targets Anti-inflammatory and Antioxidant Effects of <i>Lotus corniculatus</i> on Paracetamol-induced Hepatitis in Rats
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry