Biophysical Characterization of Fibroblast Growth Factor Homologous Factor-1b (FHF-1b): Sodium Dodecyl Sulfate Promotes Two State Folding

Title: Biophysical Characterization of Fibroblast Growth Factor Homologous Factor-1b (FHF-1b): Sodium Dodecyl Sulfate Promotes Two State Folding

Volume: 15 Issue: 2

Author(s): Vikash Kumar Dubey, Bishal Kumar Singh, Nandini Sarkar, Monu Pande, Medicherla Venkata Jagannadham, Vikash Kumar Dubey, Bishal Kumar Singh and Medicherla Venkata Jagannadham

Affiliation:

Keywords: Physiochemical studies, stability, intermediate state, FHF

Abstract: The current article describes the biophysical characterization and folding studies of fibroblast growth factor homologous factor-1b (FHF-1b) in comparison with acidic fibroblast growth factor (FGF-1). Our data indicates that FHF- 1 is significantly more stable than FGF-1. The folding mechanism of these two proteins seems to be different although they share high degree of sequence and structural similarity. FHF-1 unfolds through stable intermediate state while unfolding of FGF-1 is two-state. Interestingly, low concentration of sodium dodecyl sulfate (SDS) drives the folding pathway of FHF-1b to two-state.

Cite this article as:

Dubey Kumar Vikash, Singh Kumar Bishal, Sarkar Nandini, Pande Monu, Jagannadham Venkata Medicherla, Kumar Dubey Vikash, Kumar Singh Bishal and Venkata Jagannadham Medicherla, Biophysical Characterization of Fibroblast Growth Factor Homologous Factor-1b (FHF-1b): Sodium Dodecyl Sulfate Promotes Two State Folding, Protein & Peptide Letters 2008; 15 (2) . https://dx.doi.org/10.2174/092986608783489535