Isolation and Partial Characterization of A β-Glucuronidase of the Mollusk Pomacea sp.

Title: Isolation and Partial Characterization of A β-Glucuronidase of the Mollusk Pomacea sp.

Volume: 14 Issue: 10

Author(s): Maria Lucia Lira de Andrade, Vanessa Duarte de Morais and Joao Felipe de Sousa Filho

Affiliation:

Keywords: β- glucuronidase, glycosaminoglycans, high-performance liquid, chromatography (HPLC), Pomacea sp

Abstract: This paper studies the β-glucuronidase in the mollusk Pomacea sp. The β-glucuronidase was isolated 206-fold with a 1,5% yield and the cinetc parameters was: pH 5.0, 65°C, Km of 72 x 10-2 mM and molecular mass of 116 kDa. HPLC confirmed the purity. BaCl2 increased β-glucuronidase activity and SDS and NaH2PO4 inhibited completely.

Cite this article as:

Lira de Andrade Lucia Maria, de Morais Duarte Vanessa and de Sousa Filho Felipe Joao, Isolation and Partial Characterization of A β-Glucuronidase of the Mollusk Pomacea sp., Protein & Peptide Letters 2007; 14 (10) . https://dx.doi.org/10.2174/092986607782541097

DOI https://dx.doi.org/10.2174/092986607782541097	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305