Crystallization And Preliminary X-Ray Studies Of A Thermostable Dna Photolyase From Thermus Thermophilus Hb8

Title: Crystallization And Preliminary X-Ray Studies Of A Thermostable Dna Photolyase From Thermus Thermophilus Hb8

Volume: 8 Issue: 6

Author(s): Hirofumi Komori, Hiroshi Tsujiuchi, Ryoji Masuii, seiki Kuramitsu, Shigeyuki Yokoyama, Takehiko Shibata, Yorinao Inoue and Kunio Miki

Affiliation:

Keywords: THERMOSTABLE DNA PHOTOLYASE, THERMUS THERMOPHILUS HB8, CPD photolyase, Escherichia coli, Anacystis nidulans

Abstract: A thermostable DNA photolyase from an extremely thermophilic bacterium, Thermus thermophilus HB8 has been crystallized by the hanging drop vapor diffusion method using NH4H2PO4 as a precipitant. The hexagonal crystals were grown to a size of 0.4 mm in length. X-ray diffraction experiments show the crystals to belong to the hexagonal space group P6 5 22 or P6 1 22 with the unit cell dimensions of a=b=113.6 A, c=142.0 A. They diffract X-rays to 2.4 A resolution with synchrotron radiation.

Cite this article as:

Komori Hirofumi, Tsujiuchi Hiroshi, Masuii Ryoji, Kuramitsu seiki, Yokoyama Shigeyuki, Shibata Takehiko, Inoue Yorinao and Miki Kunio, Crystallization And Preliminary X-Ray Studies Of A Thermostable Dna Photolyase From Thermus Thermophilus Hb8, Protein & Peptide Letters 2001; 8 (6) . https://dx.doi.org/10.2174/0929866013409157