Expression of Recombinant Hepatitis B Virus Antigen Hbsag in Transgenic Plant Callus

Title: Expression of Recombinant Hepatitis B Virus Antigen Hbsag in Transgenic Plant Callus

Volume: 8 Issue: 2

Author(s): Cintia G. Kawashima, Elio H. Baba and Ekkehard Hansen

Affiliation:

Keywords: hepatitis b virus antigen hbsag, agrobacterium tumefaciens, hepatitis b virusantigen HBsAg, polypeptides, antigenicity, immunogenicity

Abstract: The coding sequence of the surface antigen HBsAg of hepatitis B virus was cloned into a plant expression vector under the control of cauliflower mosaic virus 35S promoter. Cotyledons, hypocotyls and roots of lettuce cultivar Vitória were transformed by cocultivation with Agrobacterium tumefaciens harbouring the recombinant plasmid. Lettuce callus tissue grown on selective medium was analysed and the presence of the transgene confirmed by PCR. Expression of recombinant protein HbsAg was confirmed by Western Blot.

Cite this article as:

Kawashima G. Cintia, Baba H. Elio and Hansen Ekkehard, Expression of Recombinant Hepatitis B Virus Antigen Hbsag in Transgenic Plant Callus, Protein & Peptide Letters 2001; 8 (2) . https://dx.doi.org/10.2174/0929866013409571

DOI https://dx.doi.org/10.2174/0929866013409571	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305