Abstract
The coding sequence of the surface antigen HBsAg of hepatitis B virus was cloned into a plant expression vector under the control of cauliflower mosaic virus 35S promoter. Cotyledons, hypocotyls and roots of lettuce cultivar Vitória were transformed by cocultivation with Agrobacterium tumefaciens harbouring the recombinant plasmid. Lettuce callus tissue grown on selective medium was analysed and the presence of the transgene confirmed by PCR. Expression of recombinant protein HbsAg was confirmed by Western Blot.
Keywords: hepatitis b virus antigen hbsag, agrobacterium tumefaciens, hepatitis b virusantigen HBsAg, polypeptides, antigenicity, immunogenicity
Protein & Peptide Letters
Title: Expression of Recombinant Hepatitis B Virus Antigen Hbsag in Transgenic Plant Callus
Volume: 8 Issue: 2
Author(s): Cintia G. Kawashima, Elio H. Baba and Ekkehard Hansen
Affiliation:
Keywords: hepatitis b virus antigen hbsag, agrobacterium tumefaciens, hepatitis b virusantigen HBsAg, polypeptides, antigenicity, immunogenicity
Abstract: The coding sequence of the surface antigen HBsAg of hepatitis B virus was cloned into a plant expression vector under the control of cauliflower mosaic virus 35S promoter. Cotyledons, hypocotyls and roots of lettuce cultivar Vitória were transformed by cocultivation with Agrobacterium tumefaciens harbouring the recombinant plasmid. Lettuce callus tissue grown on selective medium was analysed and the presence of the transgene confirmed by PCR. Expression of recombinant protein HbsAg was confirmed by Western Blot.
Export Options
About this article
Cite this article as:
Kawashima G. Cintia, Baba H. Elio and Hansen Ekkehard, Expression of Recombinant Hepatitis B Virus Antigen Hbsag in Transgenic Plant Callus, Protein & Peptide Letters 2001; 8 (2) . https://dx.doi.org/10.2174/0929866013409571
DOI https://dx.doi.org/10.2174/0929866013409571 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers