Isolation and Partial Characterisation of a Protein from Buck Seminal Plasma (Capra Hircus), Homologous to Spermadhesins

Title: Isolation and Partial Characterisation of a Protein from Buck Seminal Plasma (Capra Hircus), Homologous to Spermadhesins

Volume: 9 Issue: 4

Author(s): D.I. A. Teixeira, B. S. Cavada, A. H. Sampaio, A. Havt, C. Bloch Jr., M. V. Prates, F.B. M.B. Moreno, E. A. Santos, C.A. A. Gadelha, T. S. Gadelha, F.S. M. Crisostomo and V. J. F. Freitas

Affiliation:

Keywords: Buck Seminal Plasma, Capra Hircus, Spermadhesins, HPLC system, DEAE-Sephacel column

Abstract: Spermadhesins are a family of secretory proteins expressed in the male genital tract of pig, horse and bull. Their function and structure have been widely studied, especially those isolated from boar. However, there are no data concerning spermadhesins isolated from buck. Buck seminal plasma was collected and subjected to ion exchange chromatography on DEAE-Sephacel column followed by chromatography in a C18 column coupled to a HPLC system. The purification of the protein was determined by SDS-PAGE and MALDI-TOF analysis exhibiting a molecular mass of 12.5 KDa and showed to be structurally homologous to spermadhesins from boar and stallion.

Cite this article as:

Teixeira A. D.I., Cavada S. B., Sampaio H. A., Havt A., Bloch Jr. C., Prates V. M., Moreno M.B. F.B., Santos A. E., Gadelha A. C.A., Gadelha S. T., Crisostomo M. F.S. and Freitas J. F. V., Isolation and Partial Characterisation of a Protein from Buck Seminal Plasma (Capra Hircus), Homologous to Spermadhesins, Protein & Peptide Letters 2002; 9 (4) . https://dx.doi.org/10.2174/0929866023408670

DOI https://dx.doi.org/10.2174/0929866023408670	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305