Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase

Title: Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase

Volume: 10 Issue: 6

Author(s): Matsujiro Ishibashi, Tsutomu Arakawa and Masao Tokunaga

Affiliation:

Keywords: halophilic, nucleoside diphosphate kinase, refolding, charge shielding, salting-in

Abstract: Previously we reported that halobacterial nucleoside diphosphate kinase can be refolded in the presence of concentrated trimethylamine N-oxide (TMAO) as well as NaCl, indicating that enhancement of compact structure formation by TMAO is sufficient for folding. Here we showed that the refolding effect of MgCl2 is maximal at 1 M and declines to zero at 2 M, indicating that charge shielding effect of MgCl2 is offset by its salting-in effect.

Cite this article as:

Ishibashi Matsujiro, Arakawa Tsutomu and Tokunaga Masao, Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase, Protein & Peptide Letters 2003; 10 (6) . https://dx.doi.org/10.2174/0929866033478546

DOI https://dx.doi.org/10.2174/0929866033478546	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305