The Effect Of Polyols On The Reactivation Of Guanidium Chloride-Denatured Arginine Kinase From Shrimp Feneropenaeus Chinensis Muscle

Title: The Effect Of Polyols On The Reactivation Of Guanidium Chloride-Denatured Arginine Kinase From Shrimp Feneropenaeus Chinensis Muscle

Volume: 10 Issue: 2

Author(s): Zhenhang Yu and Biao Li

Affiliation:

Keywords: arginine kinase, refolding, aggregation, polyols, osmolytes

Abstract: The influence of glycerol, sorbitol, glucose and sucrose, on the refolding and reactivation courses of guanidine-denatured arginine kinase was studied. Glycerol, sucrose, and sorbitol, but not glucose, could improve the reactivation of the denatured arginine kinase, although in all cases aggregation was inhibited. Size exclusion chromatography showed that misfolded products were still formed during polyol-assisted refolding. The chemical and physical characteristics of polyols might explain the various observations.

Cite this article as:

Yu Zhenhang and Li Biao, The Effect Of Polyols On The Reactivation Of Guanidium Chloride-Denatured Arginine Kinase From Shrimp Feneropenaeus Chinensis Muscle, Protein & Peptide Letters 2003; 10 (2) . https://dx.doi.org/10.2174/0929866033479040

DOI https://dx.doi.org/10.2174/0929866033479040	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305