Influenza a Hemagglutinin C-terminal Anchoring Peptide: Identification and Mass Spectrometric Study

Larisa   V.   Kordyukova; Aleksander      L. Ksenofontov; Marina      V. Serebryakova; Tatyana      V. Ovchinnikova; Natalija      V. Fedorova; Valeria      T. Ivanova; Ludmila      A. Baratova

doi:10.2174/0929866043406850

Abstract

MALDI-TOF MS and N-terminal amino acid sequencing allowed us to identify several fragments of the C-terminal peptide of Influenza A hemagglutinin (HA) containing transmembrane domains (TMD). These fragments were detected in the organic phase of chloroform-methanol extracts from bromelain-treated virus particles. Heterogeneous fatty acylation of the C-terminus was revealed. Tritium bombardment technique might open an opportunity for 3D structural investigation of the HA TMD in situ.

Keywords: influenza a virus, hemagglutinin, fatty acylation, transmembrane domain

« Previous Next »

Rights & Permissions Print Cite

Protein & Peptide Letters

Title: Influenza a Hemagglutinin C-terminal Anchoring Peptide: Identification and Mass Spectrometric Study

Volume: 11 Issue: 4

Author(s): Larisa V. Kordyukova, Aleksander L. Ksenofontov, Marina V. Serebryakova, Tatyana V. Ovchinnikova, Natalija V. Fedorova, Valeria T. Ivanova and Ludmila A. Baratova

Affiliation:

Keywords: influenza a virus, hemagglutinin, fatty acylation, transmembrane domain

Abstract: MALDI-TOF MS and N-terminal amino acid sequencing allowed us to identify several fragments of the C-terminal peptide of Influenza A hemagglutinin (HA) containing transmembrane domains (TMD). These fragments were detected in the organic phase of chloroform-methanol extracts from bromelain-treated virus particles. Heterogeneous fatty acylation of the C-terminus was revealed. Tritium bombardment technique might open an opportunity for 3D structural investigation of the HA TMD in situ.

Export Options

Export File:

RIS (for EndNote, Reference Manager, ProCite)

BibTeX

Text

Content:

Citation Only

Citation and Abstract

About this article

Cite this article as:

Kordyukova V. Larisa, L. Ksenofontov Aleksander, V. Serebryakova Marina, V. Ovchinnikova Tatyana, V. Fedorova Natalija, T. Ivanova Valeria and A. Baratova Ludmila, Influenza a Hemagglutinin C-terminal Anchoring Peptide: Identification and Mass Spectrometric Study, Protein & Peptide Letters 2004; 11 (4) . https://dx.doi.org/10.2174/0929866043406850