Production of Recombinant Human Osteoprotegrin from Trichoplusia Ni Cells and Bombyx Mori Larvae

Title: Production of Recombinant Human Osteoprotegrin from Trichoplusia Ni Cells and Bombyx Mori Larvae

Volume: 11 Issue: 4

Author(s): Zhen Liu, Guan-zhen Yang and Xiang-Fu Wu

Affiliation:

Keywords: opg, mutant, trichoplusia ni cell, bombyx mori larvae

Abstract: Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichoplusia ni cells and Bombyx mori larvae. Native SDSPAGE and Western blot analysis revealed that OPG-Fc is present as a homodimer in Tn cells or B. mori larvae compared with OPG and OPG-280. Furthermore, the hypocalcemic effect assay showed that truncation of the C-terminal 100 residues OPG does not abolish the biological activity and Fc can be helpful in forming the OPG homodimer with improved biological activity.

Cite this article as:

Liu Zhen, Yang Guan-zhen and Wu Xiang-Fu, Production of Recombinant Human Osteoprotegrin from Trichoplusia Ni Cells and Bombyx Mori Larvae, Protein & Peptide Letters 2004; 11 (4) . https://dx.doi.org/10.2174/0929866043406904

DOI https://dx.doi.org/10.2174/0929866043406904	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305