Protein Extraction and Comparison of Stain Protocols for Analysis of Two-Dimensional Electrophoresis Gels

Title: Protein Extraction and Comparison of Stain Protocols for Analysis of Two-Dimensional Electrophoresis Gels

Volume: 11 Issue: 2

Author(s): Joana Silva, Ana Sofia Carvalho, Rui Vitorino, Pedro Domingues, Paula Teixeira and Paul Gibbs

Affiliation:

Keywords: Lactobacillus bulgaricus,, protein extraction,, 2-DE electrophoresis,, staining,

Abstract: Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.

Cite this article as:

Silva Joana, Carvalho Sofia Ana, Vitorino Rui, Domingues Pedro, Teixeira Paula and Gibbs Paul, Protein Extraction and Comparison of Stain Protocols for Analysis of Two-Dimensional Electrophoresis Gels, Protein & Peptide Letters 2004; 11 (2) . https://dx.doi.org/10.2174/0929866043478293