Expression, Purification And Characterization Of Recombinant Mouse Pc6b From Baculovirus-Infected Insect Cells

Title: Expression, Purification And Characterization Of Recombinant Mouse Pc6b From Baculovirus-Infected Insect Cells

Volume: 11 Issue: 1

Author(s): Lie Wang, Guanzhen Yang and Xiangfu Wu

Affiliation:

Keywords: btmd-mpc6b, insect cells

Abstract: we constructed a mouse PC6B truncated mutant and introduced a tag of 6 consecutive histidines at its carboxyl terminus for simple purification. Using the baculovirus expression system and standard enzymatic assay, we obtained recombinant mouse PC6B protein and with enzymatic activity.

Cite this article as:

Wang Lie, Yang Guanzhen and Wu Xiangfu, Expression, Purification And Characterization Of Recombinant Mouse Pc6b From Baculovirus-Infected Insect Cells, Protein & Peptide Letters 2004; 11 (1) . https://dx.doi.org/10.2174/0929866043478509

DOI https://dx.doi.org/10.2174/0929866043478509	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305