Efficient Immobilization of Enzymes on Microchannel Surface Through His-Tag and Application for Microreactor

Title: Efficient Immobilization of Enzymes on Microchannel Surface Through His-Tag and Application for Microreactor

Volume: 12 Issue: 2

Author(s): Masaya Miyazaki, Jun Kaneno, Susumu Yamaori, Takeshi Honda, Maria Portia P. Briones, Masato Uehara, Kazunari Arima, Kenichi Kanno, Kenichi Yamashita, Yoshiko Yamaguchi, Hiroyuki Nakamura, Hiroo Yonezawa, Masayuki Fujii and Hideaki Maeda

Affiliation:

Keywords: reversible immobilization, surface modification, microreactor, enzyme reaction

Abstract: We developed a simple immobilisation method for His-tagged enzymes on a microchannel surface. It facilitates immobilisation of protein molecule on microchannel surface through Ni-complex, using crude or purified protein solutions. By this method, we could immobilize proteins on microcapillary constantly. This method might be useful for further development of microreactor with reversibly immobilized enzymes.

Cite this article as:

Miyazaki Masaya, Kaneno Jun, Yamaori Susumu, Honda Takeshi, P. Briones Portia Maria, Uehara Masato, Arima Kazunari, Kanno Kenichi, Yamashita Kenichi, Yamaguchi Yoshiko, Nakamura Hiroyuki, Yonezawa Hiroo, Fujii Masayuki and Maeda Hideaki, Efficient Immobilization of Enzymes on Microchannel Surface Through His-Tag and Application for Microreactor, Protein & Peptide Letters 2005; 12 (2) . https://dx.doi.org/10.2174/0929866053005854