Structure-Function Relationships in Serine Protease-Bovine Pancreatic Trypsin Inhibitor Interaction

D.      Krowarsch; M.      Zakrzewska; A.   O.   Smalas; J.      Otlewski

doi:10.2174/0929866054395275

Abstract

We report our progress in understanding the structure-function relationships for the interaction between BPTI and serine proteases. We focused on extensive mutagenesis of four crucial positions from the protease binding loop of BPTI. Selected variants were characterized by determination of association constants, stability parameters and structures of protease-inhibitor complexes.

Keywords: protein-protein recognition, serine protease, canonical protein inhibitor, protein stability

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Protein & Peptide Letters

Title: Structure-Function Relationships in Serine Protease-Bovine Pancreatic Trypsin Inhibitor Interaction

Volume: 12 Issue: 5

Author(s): D. Krowarsch, M. Zakrzewska, A. O. Smalas and J. Otlewski

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Keywords: protein-protein recognition, serine protease, canonical protein inhibitor, protein stability

Abstract: We report our progress in understanding the structure-function relationships for the interaction between BPTI and serine proteases. We focused on extensive mutagenesis of four crucial positions from the protease binding loop of BPTI. Selected variants were characterized by determination of association constants, stability parameters and structures of protease-inhibitor complexes.

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Krowarsch D., Zakrzewska M., Smalas O. A. and Otlewski J., Structure-Function Relationships in Serine Protease-Bovine Pancreatic Trypsin Inhibitor Interaction, Protein & Peptide Letters 2005; 12 (5) . https://dx.doi.org/10.2174/0929866054395275