Abstract
Centrins are four-EF-hand Ca2+-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 Å. The crystals belong to space group P222 and the unit-cell parameters are a=34.442 Å, b=48.954 Å, c=72.583 Å.
Keywords: centrin, crystallization, preliminary x-ray studies
Protein & Peptide Letters
Title: Preliminary X-Ray Crystallographic Analysis of Centrin from Ciliate Euplotes octocarinatus
Volume: 12 Issue: 6
Author(s): Xiao-Jing He, Liu Li, Hai Hou, Yaqin Zhao, Aihua Liang and Zihe Rao
Affiliation:
Keywords: centrin, crystallization, preliminary x-ray studies
Abstract: Centrins are four-EF-hand Ca2+-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 Å. The crystals belong to space group P222 and the unit-cell parameters are a=34.442 Å, b=48.954 Å, c=72.583 Å.
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Cite this article as:
He Xiao-Jing, Li Liu, Hou Hai, Zhao Yaqin, Liang Aihua and Rao Zihe, Preliminary X-Ray Crystallographic Analysis of Centrin from Ciliate Euplotes octocarinatus, Protein & Peptide Letters 2005; 12 (6) . https://dx.doi.org/10.2174/0929866054395842
DOI https://dx.doi.org/10.2174/0929866054395842 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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