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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Effects of Trehalose on Pressure-Induced Inactivation of Yeast Alcohol Dehydrogenase

Author(s): Hyun Park, Gene Kidman and Dexter B. Northrop

Volume 12, Issue 6, 2005

Page: [597 - 599] Pages: 3

DOI: 10.2174/0929866054395824

Price: $65

Abstract

Isozymes of yeast alcohol dehydrogenase are slowly denatured at moderate hydrostatic pressures ( < 3 kbar). The time courses for inactivation are biphasic and both phases of both isozymes are protected by trehalose. ADH-I is slightly more barostable than ADH-II which is opposite to their thermostabilities. Trehalose at 1M extends their halflives about 6-fold at 2 kbar, pH 7.5 and 25°C. In contrast, 1M sucrose provides only 4.4-fold protection under identical conditions, a finding consistent with the superior protein stabilization of trehalose to other denaturants.

Keywords: yeast alcohol dehydrogenase, trehalose, hydrostatic pressure, protein denaturation, barostability, thermostability, surface tension


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