Screening and Purification of a Novel Trypsin Inhibitor from Prosopis juliflora Seeds with Activity Toward Pest Digestive Enzymes

Title: Screening and Purification of a Novel Trypsin Inhibitor from Prosopis juliflora Seeds with Activity Toward Pest Digestive Enzymes

Volume: 12 Issue: 6

Author(s): S. Sivakumar, O. L. Franco, P. D. Tagliari, C. Bloch Jr., M. Mohan and B. Thayumanavan

Affiliation:

Keywords: prosopis juliflora, amylase inhibitor, trypsin inhibitor, plant defense

Abstract: Several pests are capable of decreasing crop production causing severe economical and social losses. Aiming to find novel molecules that could impede the digestion process of different pests, a screening of a-amylase and trypsin-like proteinase inhibitors was carried out in Prosopis juliflora, showing the presence of both in dry seeds. Furthermore, a novel trypsin inhibitor, with molecular mass of 13,292 Da, was purified showing remarkable in vitro activity against T. castaneum and C. maculatus.

Cite this article as:

Sivakumar S., Franco L. O., Tagliari D. P., Bloch Jr. C., Mohan M. and Thayumanavan B., Screening and Purification of a Novel Trypsin Inhibitor from Prosopis juliflora Seeds with Activity Toward Pest Digestive Enzymes, Protein & Peptide Letters 2005; 12 (6) . https://dx.doi.org/10.2174/0929866054395897

DOI https://dx.doi.org/10.2174/0929866054395897	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305