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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

C-Terminus of p47phox Is Required for Interaction with Leukocyte Type Rat 12-Lipoxygenase

Author(s): Michiharu Tanabe, Hideki Kamitani, Takashi Watanabe and Olof Radmark

Volume 12, Issue 3, 2005

Page: [295 - 297] Pages: 3

DOI: 10.2174/0929866053587066

Price: $65

Abstract

In yeast two-hybrid system, rat 12-lipoxygenase (12-LO) bound to complete (390 amino acids) or the Nterminus truncated form of human p47 phox, but not to the C-terminus truncated form (residues 1-286). When glutathione S-transferase fused human p47phox was added to an in vitro 12-LO enzyme activity assay, formation of 12- hydroperoxyeicosatetraenoic acid was reduced significantly compared to the C-terminus truncated form. These results indicate that C-terminus of p47phox is important for its interaction to rat 12-LO.

Keywords: lipoxygenase, phagocyte, yeast two-hybrid, nadph oxidase, chaperone, dnak


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