Abstract
Eukaryotic translation initiation factor 5A (eIF-5A) is universally found in all eukaryotic cells. It is the only protein in nature known to contain the unusual amino acid hypusine, a post-translationally modified lysine. Recombinant human eIF-5A was crystallized by the hanging-drop vapor diffusion method. Crystals were grown at 291K using (NH4)2SO4 as precipitant. Diffraction data were obtained to a resolution of 2.7Å from a single frozen crystal belonging to space group C2, with unit-cell parameters a=147.1Å, b=60.4Å, c=76.4Å, β=92.4 . There are more than three molecules per asymmetric unit.
Keywords: Crystallographic Analysis, Human Eukaryotic Translation Initiation Factor 5A (eIF-5A), (NH4)2SO4, amino acid hypusine
Protein & Peptide Letters
Title: Crystallization and Preliminary Crystallographic Analysis of Human Eukaryotic Translation Initiation Factor 5A (eIF-5A)
Volume: 12 Issue: 7
Author(s): Yuna Sun, Xuemei Li, Beili Wu, Ping Sun and Zihe Rao
Affiliation:
Keywords: Crystallographic Analysis, Human Eukaryotic Translation Initiation Factor 5A (eIF-5A), (NH4)2SO4, amino acid hypusine
Abstract: Eukaryotic translation initiation factor 5A (eIF-5A) is universally found in all eukaryotic cells. It is the only protein in nature known to contain the unusual amino acid hypusine, a post-translationally modified lysine. Recombinant human eIF-5A was crystallized by the hanging-drop vapor diffusion method. Crystals were grown at 291K using (NH4)2SO4 as precipitant. Diffraction data were obtained to a resolution of 2.7Å from a single frozen crystal belonging to space group C2, with unit-cell parameters a=147.1Å, b=60.4Å, c=76.4Å, β=92.4 . There are more than three molecules per asymmetric unit.
Export Options
About this article
Cite this article as:
Sun Yuna, Li Xuemei, Wu Beili, Sun Ping and Rao Zihe, Crystallization and Preliminary Crystallographic Analysis of Human Eukaryotic Translation Initiation Factor 5A (eIF-5A), Protein & Peptide Letters 2005; 12 (7) . https://dx.doi.org/10.2174/0929866054696091
DOI https://dx.doi.org/10.2174/0929866054696091 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers