Expression and Purification of Soluble Non-Fusion Vasostatin in Escherichia coli

Xiaoping      Wu; Xiaokun      Li; Zhijian      Su; Qing      Zheng; Hua      Xu; Sixian      Wu; Ya      Feng; Wen      Zhao

doi:10.2174/0929866054696163

Abstract

Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.

Keywords: vasostatin, soluble expression, purification

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Protein & Peptide Letters

Title: Expression and Purification of Soluble Non-Fusion Vasostatin in Escherichia coli

Volume: 12 Issue: 7

Author(s): Xiaoping Wu, Xiaokun Li, Zhijian Su, Qing Zheng, Hua Xu, Sixian Wu, Ya Feng and Wen Zhao

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Keywords: vasostatin, soluble expression, purification

Abstract: Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.

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Wu Xiaoping, Li Xiaokun, Su Zhijian, Zheng Qing, Xu Hua, Wu Sixian, Feng Ya and Zhao Wen, Expression and Purification of Soluble Non-Fusion Vasostatin in Escherichia coli, Protein & Peptide Letters 2005; 12 (7) . https://dx.doi.org/10.2174/0929866054696163