Expression and Purification of Soluble Non-Fusion Vasostatin in Escherichia coli

Title: Expression and Purification of Soluble Non-Fusion Vasostatin in Escherichia coli

Volume: 12 Issue: 7

Author(s): Xiaoping Wu, Xiaokun Li, Zhijian Su, Qing Zheng, Hua Xu, Sixian Wu, Ya Feng and Wen Zhao

Affiliation:

Keywords: vasostatin, soluble expression, purification

Abstract: Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.

Cite this article as:

Wu Xiaoping, Li Xiaokun, Su Zhijian, Zheng Qing, Xu Hua, Wu Sixian, Feng Ya and Zhao Wen, Expression and Purification of Soluble Non-Fusion Vasostatin in Escherichia coli, Protein & Peptide Letters 2005; 12 (7) . https://dx.doi.org/10.2174/0929866054696163

DOI https://dx.doi.org/10.2174/0929866054696163	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305