Selection of a Catalytically Active Enzyme In Vivo Via Phage Display of its Product

Title: Selection of a Catalytically Active Enzyme In Vivo Via Phage Display of its Product

Volume: 2 Issue: 6

Author(s): T. Taki, S. Fujita and K. Taira

Affiliation:

Keywords: biotin protein ligase, in vivo selection, phage display, enzyme catalysis

Abstract: We have developed a general strategy for the selection of genes that encode active enzymes. In our strategy, the product of a catalytic reaction in vivo is displayed on secreted phage via the g8p protein. The subsequent affinity-capture of the product in vitro results in enrichment of the gene that encodes an active enzyme. In a model study, we succeeded in the selection of a gene for biotin protein ligase.

Cite this article as:

Taki T., Fujita S. and Taira K., Selection of a Catalytically Active Enzyme In Vivo Via Phage Display of its Product, Letters in Drug Design & Discovery 2005; 2 (6) . https://dx.doi.org/10.2174/1570180054771482

DOI https://dx.doi.org/10.2174/1570180054771482	Print ISSN 1570-1808
Publisher Name Bentham Science Publisher	Online ISSN 1875-628X