Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification

Title: Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification

Volume: 14 Issue: 4

Author(s): Hayan Lee, Kyung-Nam Kim and Young Kee Chae

Affiliation:

Keywords: Taq DNA polymerase, long amplicons, primers, reaction buffer

Abstract: We tested the ability of Taq DNA polymerase (Taq) to amplify long DNA fragments and showed that, if the conditions were set properly, Taq could successfully perform the “long PCR” up to 24 kb. The conditions include: (1) longer primers, (2) a 2-step cycling, and (3) a “long buffer.” We propose that the most important requirements are the survival rate of Taq at high temperatures and that of the primers against the 5 to 3 exonuclease activity of Taq.

Cite this article as:

Lee Hayan, Kim Kyung-Nam and Kee Chae Young, Reevaluating the Capability of Taq DNA Polymerase: Long PCR Amplification, Protein & Peptide Letters 2007; 14 (4) . https://dx.doi.org/10.2174/092986607780363934

DOI https://dx.doi.org/10.2174/092986607780363934	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305