Crystallization and Preliminary X-Ray Studies of the DNA-Binding Domain of Hepatocyte Nuclear Factor-6α Complexed with DNA

Title: Crystallization and Preliminary X-Ray Studies of the DNA-Binding Domain of Hepatocyte Nuclear Factor-6α Complexed with DNA

Volume: 13 Issue: 5

Author(s): Daisuke Iyaguchi, Min Yao, Nobuhisa Watanabe, Jun Nishihira and Isao Tanaka

Affiliation:

Keywords: HNF-6, Transcription regulator, Homeobox, ONECUT, Crystallization

Abstract: Hepatocyte nuclear factor 6 (HNF-6)/OC-1, a part of liver-enriched transcription factor, controls pancreas and liver development and regulates expression of several hepatic genes. DNA-binding region of HNF-6α bound to a 14-mer DNA fragment has been crystallized by the hanging drop vapor diffusion method. The crystals belong to space group P2 with unit cell parameters of a = 73.0 Å, b = 39.0 Å, c = 106.5 Å, β= 107.6 . X-ray diffraction data were collected to 2.0 Å resolution.

Cite this article as:

Iyaguchi Daisuke, Yao Min, Watanabe Nobuhisa, Nishihira Jun and Tanaka Isao, Crystallization and Preliminary X-Ray Studies of the DNA-Binding Domain of Hepatocyte Nuclear Factor-6α Complexed with DNA, Protein & Peptide Letters 2006; 13 (5) . https://dx.doi.org/10.2174/092986606776819592

DOI https://dx.doi.org/10.2174/092986606776819592	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305