Quasi-Life Self-Organizing Systems: Based on Ensembles of Succinylated Derivatives of Interferon-Gamma

A.      Martynov; B.      Farber; S.      Farber

doi:10.2174/092986711796504574

Abstract

Research has been conducted on the chemical and biological properties of combinatorial succinylated derivatives of interferon-γ with various levels of acylation, which create quasi-life, self-organizing ensembles. As a result of the research, it has been established that acylation by succinic anhydride of two lysines in the structure of interferon-γ (Gammaferon) leads to both an increase in its affinity to cell receptors and a decrease in the time of maximum effect from 48 hours to 15 minutes. Moreover, treatment of cells with these interferon ensembles led to the shielding of 100% of the cells after a 15-minute incubation period, whereas native interferon shielded no more than 80% of the cells after 48 hours. Other ensembles also protected cells from viral action, but this protective effect did not exceed two hours in duration. The ensemble of succinylated interferon-γ with two modified lysines may hold promise for the treatment of severe viral infections with fast courses, such as influenza and the diseases caused by the Ebola, Marburg, SARS, and other viruses.

Keywords: Antiviral, biological activity, cell protection, interferon-γ, self-organizing systems, succinylation, supramolecular ensembles, interferon ensembles, Gammaferon, acylation