Purification, and Properties of a Bovine Uricase

Title: Purification, and Properties of a Bovine Uricase

Volume: 13 Issue: 4

Author(s): Muhammad I. Rajoka, Munazza Mehraj, Muhammad W. Akhtar and Muhammad A. Zia

Affiliation:

Keywords: Bovine uricase, enthalpy, entropy, kinetics and thermodynamics, temperature inactivation

Abstract: Uricase from bovine kidney, purified to homogeneity level, had a molecular weight of 70 kDa. The apparent Km and Vmax values for uric acid hydrolysis were 0.125 mM and 102 IU mg-1 protein respectively. The activation energy requirement for uric acid hydrolysis by uricase and inactivation of enzyme were 11.6 and 14.5 kJ/M respectively. Both enthalpy (Δ H*) and entropy of activation (Δ S*) for uricase activity were lower than those reported for some thermostable enzymes.

Cite this article as:

Rajoka I. Muhammad, Mehraj Munazza, Akhtar W. Muhammad and Zia A. Muhammad, Purification, and Properties of a Bovine Uricase, Protein & Peptide Letters 2006; 13 (4) . https://dx.doi.org/10.2174/092986606775974366

DOI https://dx.doi.org/10.2174/092986606775974366	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305