Expression of Active α-N-Acetylglucosaminidase/TAT Chimerae in Cultured Spodoptera frugiperda Cells

Judith   C.   Bandsmer; Tessa   N.   Campbell; Ian      Cheyne; Francis   Y.M.   Choy

doi:10.2174/092986606775974456

Abstract

We examined the production and secretion of fusion constructs containing α-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibility of utilizing TAT/α-Nacetylglucosaminidase chimerae in enzyme replacement therapy.

Keywords: TAT, α-N-acetylglucosaminidase, mucopolysaccharidosis, Sanfilippo, protein expression

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Protein & Peptide Letters

Title: Expression of Active α-N-Acetylglucosaminidase/TAT Chimerae in Cultured Spodoptera frugiperda Cells

Volume: 13 Issue: 4

Author(s): Judith C. Bandsmer, Tessa N. Campbell, Ian Cheyne and Francis Y.M. Choy

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Keywords: TAT, α-N-acetylglucosaminidase, mucopolysaccharidosis, Sanfilippo, protein expression

Abstract: We examined the production and secretion of fusion constructs containing α-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibility of utilizing TAT/α-Nacetylglucosaminidase chimerae in enzyme replacement therapy.

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Bandsmer C. Judith, Campbell N. Tessa, Cheyne Ian and Choy Y.M. Francis, Expression of Active α-N-Acetylglucosaminidase/TAT Chimerae in Cultured Spodoptera frugiperda Cells, Protein & Peptide Letters 2006; 13 (4) . https://dx.doi.org/10.2174/092986606775974456